EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

PATHOLOGICAL AND MOLECULAR INVESTIGATION OF DUCK VIRAL HEPATITIS (DVH) IN KALIOBEYA GOVERNORATE, EGYPT

*Nora M. Elseddawy and Heba M. Abdel-Ghany

ABSTRACT

One thousand, 14 day-old Pekin ducklings suffered from depression, eye closing, watery diarrhea and the affected bird fall on their side and kick spasmodically with head stretched upward and backward (opsithotonos position) with mortality rate reached up to 80% reared in kaliobeya governorate were the subject of our investigation. Specimens from different organs including liver spleen, lungs, intestine, heart, gizzard, proventriculus, pancreas, brain and bursa were collected and subjected to the pathology The collected specimen were fixed in 10% neutral buffered formalin solution, dehydrated in gradual alcohol (70-100%), cleared in xylene and embedded in paraffin. Five microns thickness paraffin sections were prepared and stained with hematoxylin and eosin (HE) dyes then examined microscopically. Some of the collected specimens were stored frozen at -20°C to be subjected Reverse - transcriptase polymerase chain reaction (RT-PCR) for the detection of Duck viral hepatitis (DVH). Macroscopically, the liver was enlarged, yellowish brown in color with hemorrhagic spots on its surface, the kidneys, spleen and lungs were enlarged and congested. The brain showed congestion in meninges with hemorrhagic patches beside congestion of intestinal mesentery was observed. Hemorrhage, congestion, cell swelling, necrosis with infiltration of heterophiles and lymphocytes were observed microscopically in the examined organs. PCR technique was positive for Duck viral hepatitis and it was the best tool to diagnose the disease.

Keywords: DVH, poultry, Picornaviridae, opsithotonos, PCR.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing