EVALUATION OF ANTIBACTERIAL POTENTIAL OF CANAVALIA GLADIATA (JACQ.) DC.

Tayade S. N., More K. C.* and Gawande P. A.

ABSTRACT

The present investigation were carried out to evaluate the antimicrobial potential of leaves and seed extracts of Canavalia gladiata against human pathogens viz Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis by using disc diffusion method. The five different solvents viz petroleum ether, chloroform, acetone, ethanol, and methanol were taken for extraction of complete phytoconstituents. The zone of inhibition of each extract against pathogenic bacteria was evaluated. The significant zones of inhibition was observed in leaves extracted with ethanol were found to be 20.0 mm against Bacillus subtilis followed by seed chloroform extracts i. e 14.0mm against Bacillus subtilis. Leaves acetonic extract shows 10.5 mm and 10.00 mm zone of inhibition against Pseudomonas aeruginosa and Staphylococcus aureus respectively, whereas, ethanolic extract of leaves exhibits 11.5 mm zone of inhibition against Staphylococcus aureus. Moreover, chloroform seed extracts found to be 11.0 mm and 10.00 mm zone of inhibition against Bacillus sbstilis and Pseudomonas aeruginosa respectively. Acetonic seed extracts exhibits 12.00 mm zone of inhibition against Bacillus subtilis. The ethanolic seed extracts exhibits 11.5mm zone of inhibition against Staphylococcus aureus. Methanolic seed extracts found to be 11.00mm and 10.00mm zone of inhibition against Staphylococcus aureus and Pseudomonas aeruginosa respectively.

Keywords: Antibacterial; Canavalia gladiata; Bioactive Phytoconstituents.