EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DOLUTEGRAVIR AND LAMIVUDINE IN TABLET DOSAGE FORM BY RP-HPLC

Dr. Sonali P. Mahaparale* and Jayraj U. Deshmukh

ABSTRACT

A Novel reversed phase high performance liquid chromatographic method was developed and validated for the simultaneous determination of human immune deficiency virus drug Dolutegravir (DGV) and Lamivudine(LMD) present in formulation known as Dovato which consists 50 mg of DGV and 300 mg LMD. Chromatographic separation achieved isocratically on thermo C18 column (5μm, 150mm x 4.60mm) and Acetonitrile Water(pH 5) in the ratio of 50:50 (v/v) as the mobile phase, at a flow rate of 0.8 ml/min. Detection was carried out at 267 nm. The retention times for DGV and LMD was found to be 2.56 min and 6.7 min respectively. Parameters like linearity, precision, accuracy, recovery, specificity and ruggedness are studied as reported within the ICH guidelines. The method was linear in the concentration range of 25-75 μg/ml for (DGV) and 150-450 μg/ml for (LMD). Correlation coefficient of Dolutegravir and Lamivudine was found to be 0.999 and 0.998 respectively. Developed method was found to be novel, accurate, precise, selective and rapid for simultaneous estimation of DGV and LMD.

Keywords: Reverse Phase, Isocratic elution, Linearity, Chromatographic separation.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing