METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF NETARSUDIL & LATANOPROST BY RP-HPLC METHOD
Lamba Venkata Ramana*, Dr. Devanaboyina Narendra and Gadi Vijaya Lakshmi
A simple, Accurate, precise method was developed for the simultaneous estimation of the Netarsudil and Latanoprost in Opthalmic solution dosage form. Chromatogram was run through Std Agilent C18 150 x 4.6 mm, 5. Mobile phase containing Buffer KH2PO4: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1ml/min. Buffer used in this method was Potassium dihydrogen phosphate. Temperature was maintained at 30°C. Optimized wavelength selected was 220nm. Retention time of Netarsudil and Latanoprost were found to be 2.875min and 4.106min. %RSD of the Netarsudil and Latanoprost were and found to be 0.9 and 0.9 respectively. %Recovery was obtained as 100.46% and 100.20% for Netarsudil and Latanoprost respectively. LOD, LOQ values obtained from regression equations of Netarsudil and Latanoprost were 0.08, 0.25 and 0.04, 0.12 respectively. Regression equation of Netarsudil is y = 62952x + 4328.1 and y = 43163x + 510.88 of Latanoprost. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
Keywords: Netarsudil, Latanoprost, RP-HPLC.
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