EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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 ICV - 79.57

Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF RITONAVIR, OMBITASVIR & PARITAPREVIR BY RP-HPLC METHOD

Medisetti Pravallika*, Dr. Devanaboyina Narendra and Gadi Vijaya Lakshmi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Ritonavir, Ombitasvir and Paritaprevir in tablet dosage form. Chromatogram was run through Ascentis c18 150 x 4.6 mm, 5. Mobile phase containing Acetonitrile and Water in the proportion of 60:40 was pumped through column at a flow rate of 0.8ml/min. Temperature was maintained at 30°C. Optimized wavelength for Ritonavir, Ombitasvir and Paritaprevir was 265nm. Retention time of Ritonavir, Ombitasvir and Paritaprevir Were found to be 2.147 min; 2.732 min and 3.790 min. %RSD of system precision for Ritonavir, Ombitasvir and Paritaprevir were and found to be 0.4, 1.0 and 1.0 respectively. %RSD of method precision for Ritonavir, Ombitasvir and Paritaprevir were and found to be 0.5, 0.4 and 0.8 respectively. % recovery was obtained as 100.30%, 100.19% and 100.15% for Ritonavir, Ombitasvir and Paritaprevir respectively. LOD, LOQ values are obtained from regression equations of Ritonavir, Ombitasvir and Paritaprevir were 0.14ppm, 0.44ppm, 0.06ppm, 0.19ppm and 0.42ppm, 1.28ppm respectively. Regression equation of Ritonavir was y = 28427x + 10360, Ombitasvir was y = 8648.x + 422.2 and of Paritaprevir was y = 39168x + 17464. Retention times are not as much as different techniques so the method developed were basic and conservative that can be received in standard Quality control test in Industries. Retention times are decreased so the method developed basic and conservative embraced in general Quality control test in Industries.

Keywords: Ritonavir. Ombitasvir and Paritaprevir, RP-HPLC.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )