EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

A RAPID - CHEMO METRICS ASSISTED RP-HPLC METHOD WITH PDA DETECTION FOR THE SIMULTANEOUS ESTIMATION OF OFLOXACIN AND NIMORAZOLE IN PURE AND PHARMACEUTICAL FORMULATION

P. Giriraj, T. Sivakkumar*

ABSTRACT

A simple, rapid, sensitive, cost effective and reproducible chemo metrics assisted RP-HPLC method was developed, optimized and validated for the simultaneous estimation of the Ofloxacin and Nimorazole in pure and pharmaceutical formulation. The developed method was optimized by using Central composite design (CCD) in resoponse surface methodology (RSM). Based on the trial and error, percentage of tetrahydrofuran in mobile phase, flow rate and buffer molarity were selected as factors. Resolution (Rs2) and retention time (tR2) were used for the estimation of system response during the optimization procedure. Derringer’s desirability function was used to concurrently optimize the selected two responses. Separation of ofloxacin and nimorazole was achieved on phenomenex C18 column (150 X 4.6 mm i.d, 5μ particle size) with a mobile phase consisting of 25% of tetrahydrofuran and 75% of Phosphate buffer (25mM) was delivered at a flow rate of 1.2mL/min and photodiode array detection at 235nm. The method was found to be linear over the concentration range of 20 – 60μg/mL for ofloxacin and 50 – 150μg/mL for nimorazole with their correlation coefficient values of 0.9995 and 0.9997 respectively. LOD and LOQ were found to be 20.62ng/mL and 62.49ng/mL for ofloxacin, 188.65ng/mL and 571.68ng/mL for nimorazole. The % RSD value of accuracy and precision study was found to be less than 2%. The proposed method is simple, rapid, sensitive, cost effective and reproducible. Hence

Keywords: this method can be used for routine quality control analysis of ofloxacin and nimorazole in pure and pharmaceutical dosage form


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )