HUMAN PAPILLOMA VIRUSES 16 AND 18 IN SUDANESE ORAL SQUAMOUS TISSUES
Asim Awad Mohammed Ahmed*, Mohammed Elimam Ahamed Mohammed, Dafalla Omer Abuidris Elmustafa, Kanan Sanhouri, Ali Idris, Mohamed E. Abdelwadoud6, Abdelbasit M. I. Hussein, Dr. Hisham Altyb and Adil Abdelrahim Mohammed Yousif
Human papilloma viruses 16 and 18 (HPV-16 and HPV-18) are well known to play some roles in cervical and oral squamous cell carcinomas. The aim of this study was to investigate the expression of HPV-16 and HPV-18 in normal and cancerous oral squamous tissues using the immunohistochemistry (IHC) and polymerase chain reaction (PCR) techniques. Fifty one oral squamous carcinoma tissues and 49 normal oral squamous tissues were investigated for HPV-16 and HPV-18 detection. The immunohistochemistry results showed that the HPV-16 was positive in 2.04% of the normal tissues and in 9.8% of the cancerous tissues while the HPV-18 was positive in 8.2% and 17.6% of the normal and cancerous tissues, respectively. There was insignificant variation between the IHC results of the normal and cancerous tissues. 4.08% and 5.9% of the normal and cancerous tissues were positive for HPV-16 PCR, respectively. Concerning the PCR results of the HPV-18, all the normal and cancerous tissues were negative for HPV-18. There was insignificant variation between the HPV-16 detection by IHC and PCR while the HPV-18 detection by IHC was significantly sensitive compared to the PCR. The odd ratios of IHC for HPV-16 and HPV-18 showed that being positive was more likely to be OSCC 5.22 and 2.42 times, respectively compared to being negative for them. There was an insignificant variation between the expression of HPV-16 and HPV-18 in the normal and cancerous oral squamous tissues of the Sudanese subjects. The IHC was significantly sensitive for the detection of HPV-18 than the PCR.
Keywords: OSCC, IHC, PCR, HPV-16, HPV-18.
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