BIOPOLYMER FORM CESTRUM NOCTURNUM FLOWER: ISOLATION, CHARACTERIZATION AND ITS UTILIZATION IN PREPARATION OF PHENYTOIN LOADED BIO-NANOSUSPENSION

Sushant Kumar*, N. V. Satheesh Madhav, Anurag Verma and Kamla Pathak

ABSTRACT

The aim of this research was to formulate phenytoin loaded bio-nanosuspension for targeting to brain via ear using novel isolated biopolymer form Cestrum nocturnum flower. The significance of this research was to isolate the bio-polymer by using an economical and simple procedure. Bio-polymer was subjected for various physicochemical characterizations with spectral analysis like UV, FT-IR, mass and H-NMR, DSC was done. The phenytoin loaded bio-nanosuspension (PCN1-PCN8) were prepared by bath sonication method using isolated stabilizer cum retardant novel bio-polymer in different ratio. Various formulations were evaluated for different parameters like pH study, dispersibility study, entrapment efficacy, zeta particle size, zeta potential, In-vitro release study, stability studies and In-Vivo study. The formulation PCN8 was found to be the best formulation having t50% of 17.06 hours and t80% of 29.06 hours with r2 value of 0.9899. The best formulation PCN8 showed up to 90.87% drug release over 36 hours. According to the release kinetic study the best fit model was found to be Korsmeyer Peppas and the mechanism of drug release was found to be Anomalous transport. The isolated biopolymer was found to have a promising, novel inbuilt stabilizer cum retardant polymeric properties for developing stable phenytoin loaded bionanosuspension. The formulated bionanosuspension was found to be safe and compatible for targeting to brain via ear.

Keywords: Biopolymer, Bio-nanoparticles, bionanosuspension, Phenytoin, Nanosizing, drug targeting.