STUDIES ON THE RHIZOSPHERE MYCOFLORA OF MUSTARD

*Sakshi Tiwari and Pragati Bailwal

ABSTRACT

Rhizospheric soil sample of mustard (Brassica sp.) was collected from the cultivated land area near Gurukul Kangri University, Haridwar. It was reported that Trichodema and Aspergillus were dominant in mustard rhizosphere. During the growth rate measurement (colony diameter method and dry mycelial weight method) of the isolates, it was observed that Trichoderma grew at a faster rate whereas Aspergillus grew at much lower rate. During the study of effect of temperature on growth of the isolates, it was observed that all the isolates were able to grow at temperature ranges (15º, 25º and 35ºc). Hence they are mesophiles. While studying the effect of pH on growth of the isolates, it was observed that Trichoderma is acidophile whereas Aspergillus is alkalophile. Trichoderma and Aspergillus has potential to solubilize phosphate. Antifungal sensitivity was determined by Poisoned Food Technique and it was found that nystatin is most effective among all antifungal drugs.

Keywords: Fungi, Rhizosphere and Mustard.