ASSESSMENT OF HEMATOLOGICAL MARKERS USING THYMOQUINONE AS EXPERIMENTAL AND SILYMARIN AS STANDARD DRUG AGAINST HEPATIC INSULT CAUSED BY FIRST LINE ANTI TUBERCULOSIS DRUGS

Muhammad Waqas*, Shoaib Asif, Muhammad Masood, Zaman Hayat, Waqar Siddique, Farhan Asghar, Rabbia Tariq, Azka Musharaf and Imran Waheed

ABSTRACT

Gram negative rod Mycobacterium tuberculosis is the underlying causative agent for the progress of life threatening disease tuberculosis. First line antituberculosis drugs are main choice for physician to counter this dangerous disease, by producing promising effect to counter Mycobacterium tuberculosis, the selected group of drugs produce hepatotoxicity. In this study Thymoquinone, main constituent of Nigella sativa was used as experimental drug while silymarin as standard drug. The thymoquinone found to produce hepatoprotective effects in rabbits treated anti tuberculosis drugs. Thymoquinone showed remarkable result and persisted successful in achieving level of ALT, which is 17.09 (SD±1.39) close to the usual mean value of 18.96, results were found significant (P value < 0.005). Thymoquinone in both 40mg and 20 mg dose found effective but the result by TQ 40mg were extraordinary by achieving the level to (50.27 SD±5.88) very near to the normal mean level of (48.10) in contrast with standard drug silymarin (56.96 SD± 8.36). One more intense effect was observed on day 7, when TQ cut the value down from (54.18) on same day silymarin (70.00) was unable to change the level which prove the short onset of action of thymoquinone as compare to standard drug. P value < 0.005 were found statically significant.

Keywords: Mycobacterium Tuberculosis, Silymarin, Thymoquinone, ALT, AST.