GAS CHROMATOGRAPHY MASS SPECTROMETRY PROFILING, PHARMACOLOGICAL ACTIVITIES OF ETHANOL FLOWER EXTRACT OF BRASSICA OLERACEA VAR. ITALICA-BROCCOLI

Arumugam.P*, Saraswathi.K, Dhivya.M, Akshaya.J, Monica Joicy.C, Sivaraj.C

ABSTRACT

There are over a two lakh twenty thousand phytochemicals out of which fruits and vegetables have hundred different phytochemicals and two hundred and fifty thousand species of plants exists on this planet containing different kinds of secondary metabolites, known as Phytochemicals.Natural antioxidants having low or no side effects are used in prevention of diseases plants produce secondary metabolites, most of the antioxidant compounds are flavonoids, phenols ,anthocyanins, carotenoids are used to overcome the oxidative stress caused by reactive oxygen species.Current research studies were carried out for evaluating the antioxidant and antibacterial activities of ethanol flower extract of Brassica oleracea var. italica-Broccoli. Antioxidant activities such as DPPH˙ radical, Superoxide (O2.-) radical, ABTS●+ radical cation, phosphomolybdenum reduction and Fe3+ reduction were carried out for ethanol flower extract of Brassica oleracea var. italica-Broccoli. The maximum DPPH˙ radical and Superoxide (O2.-) radical scavenging activities were 54.39±0.42% and 73.07±0.29% at 120 μg/mL concentration and the IC50 values were 80.67 μg/mL and 57.59 μg/mL concentrations respectively. The maximum ABTS●+ radical cation scavenging activity was 87.63±0.42% at 12 μg/mL concentration and the IC50 value was 6.50 μg/mL concentration respectively. The maximum Mo6+ reduction and Fe3+ reduction were 90±0.26% and 52±0.31% at 120 μg/mL concentration and the RC50 values were 15.15 μg/mL and 104.16 μg/mL concentrations respectively. The antibacterial activity of ethanol flower extract of Brassica oleracea var. italica-Broccoli showed maximum zone of inhibition of 23 mm for Escherichia coli at 750 μg/mL concentration. Oleic acid, Quinoxaline, 2-isopropyl-3-phenyl, 4-oxide, n-Hexadecanoic acid were found to be the active compounds detected from GCMS analysis.

Keywords: Superoxide (O2.-) radical, ABTS?+ radical cation, Phytochemicals, GCMS.