EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

INVITRO CYTOTOXIC EFFECT AND APOPTOSIS INDUCTION OF HYDROALCOHOLIC EXTRACT OF BOERHAAVIA DIFFUSA LINN AGAINST HUMAN CANCER CELL LINES

*G. Nalini, N. Chidambaranathan, S. A. Syed Ibrahim and K. Marihrishnaa

ABSTRACT

The aim of the present study is to evaluate the effect of in-vitro anticancer activity of the Hydroalcoholic extract of Boerhaavia diffusa linn against MCF-7 breast cancer & HCT-116 colon cancer cell lines. The viability of cells were evaluated by direct observation of cells by Inverted phase contrast microscope and followed by MTT assay method. The percentage of cell viability was found to be 97% at 6.25μg/ml on HCT-116 which decrease to 28% with increase in concentration of extract at 100μg/ml. Similarly for MCF-7 cell line, cell viability shows 70% at a concentration of 6.25μg/ml that reduced to 40% at 100μg/ml. The effect of hydro-alcoholic extracts of Boerhaavia diffusa linn on cell morphology of MCF-7 and HCT-116 cells was examined using phase-contrast microscope. Treatment with plant extract showed typical morphological changes on MCF-7 & HCT-116 exhibited condensed chromatin, apoptotic bodies with membrane blebs and cell shrinkage. Apoptosis changes and chromatin condensation were also determined by fluorescent microscope. The results indicate that Boerhaavia diffusa is a considerable source of natural bioactive substances with antiproliferative activity on HCT-116 & MCF-7 cells & significant morphological effects.

Keywords: Antiproliferative, MTT assay, apoptosis, chromatin condensation, Boerhaavia diffusa linn.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database