TO STUDY AND EVALUATION OF NEUROPROTECTIVE POTENTIAL OF BENIDIPINE IN FERRIC CHLORIDE INDUCED POST TRAUMATIC EPILEPSY IN MICE

Rinki*, Shiva Yadav, Yashpal Singh and Vivek

ABSTRACT

Background:- Post-traumatic epilepsy (PTE) is a form of symptomatic epilepsy defined by the presence of recurrent seizures secondary to traumatic brain injury, presenting with variable latency of onset and course. Traumatic brain injury (TBI) greatly increases the risk for a number of mental health problems and is one of the most common causes of medically intractable epilepsy in humans. Objective: To study and evaluation of neuroprotective potential of benidipine in post traumatic epilepsy in mice. Methods: In present study, post traumatic epilepsy was induced by intracortical injection of ferric chloride. Mice were anesthetized with intraperitoneal injection of ketamine (80mg/kg) and xylazine (20 mg/kg). A midline sagittal incision of 1cm was made on scalp. Bregma and lambda were located and the centre point between the two was marked. The skull was exposed and a burr hole was made in the location over the left sensorimotor cortex, 1mm posterior and 2mm lateral to bregma. Ferric chloride solution (100mM, 5μl) was injected 2mm below skull at rate of 1μl/min through a hamilton syringe. Result:- In present study, PTE was induced by ferric chloride induced model of post-traumatic epilepsy & benidipine 9mg/kg; p.o. showed most neuroprotective potential improving behavioral parameters ( open field test, rota rod test, actophotometer test and anhedonia test) and oxidative damage. Conclusion: This study demonstrated the neuroprotective potential of benidipine in post traumatic epilepsy in mice.

Keywords: Epilepsy, Post-traumatic epilepsy (PTE), Traumatic brain injury.