EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

INVIVO AND EXVIVO ANTIOXIDANT ACTIVITIES OF PERSEA AMERICANA EXTRACTS ON ALBINO WISTAR RATS

Iloh E. S., C. S. Nworu, *M. A. Omoirri, N. U. Madubogwu, B. C. Ugwu

ABSTRACT

For many years now, plants and plant products have been used in traditional medicine for treatment of infectious diseases. This study investigated the anti-oxidant activity of Persia americana extract under invivo and ex vivo conditions. The antioxidant activity of the leaf extract/fractions of P. americana was carried out in vivo using the 1, 1-diphenyl-2-picryl-hydraxyl (DPPH) assay. A total of sixty five (65) healthy adult Albino rats of either sexes, weighing between 200 - 250 g were procured and housed in the animal house of the Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra state, Nigeria. They were maintained under standard environmental conditions with free access to standard diet and water ad libitum. They were then grouped into 13 groups of five rats per group. For the aqueous extract unit, group 1 received 10ml/kg of distilled water, while group 2 received 50mg/kg of diclofenac. Groups 3, 4 and 5 received 50mg/kg, 150mg/kg and 400mg/kg body weights of crude extract respectively. Fractions extract unit received 10ml/kg of distilled water (Group 1, control) and 50mg/kg of diclofenac (Group 2). Groups 3 and 4 were then given 150mg/kg of ethyl acetate fraction and 400mg/kg of ethyl acetate fraction respectively, while groups 5, 6, 7 and 8 got 150mg/kg, 400mg/kg of n-hexane fraction and 150mg/kg of butanol fractions respectively. Phytochemical evaluation of the plant confirmed the presence of alkaloids, flavonoids, saponins, tannins, steroids, terpenoids, glycosides, carbohydrates, proteins, and coumarins. In the antioxidant assay, the crude extract and fractions of the P. americana leaves were administered to adult albino rats at variable doses. Various degrees of antioxidant activities at the concentration range of 31.25 -500 µg/mL were observed, with inhibition ranging from 4.2 – 41.8%. The crude extract showed best antioxidant activity compared with the fractions with IC50 = 587 µg/mL. Thus, the results of this study investigated the antioxidant properties of P. americana leaves extract, providing scientific information that validates the ethnobotanical use of P. americana leaves in disease management. Findings of this study also reveal that potentially P. americana could be a source of pharmacologically active compounds of pharmaceutical importance.

Keywords: Persia Americana, Antioxidant, Phytochemicals, fractions.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )