A REVIEW ON DEVELOPMENT AND VALIDATION OF ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY FOR ESTIMATION OF DRUG IN PHARMACEUTICAL DOSAGE FORM.

Pradnya G. Shelke*, Vijay S. Wakale and Ganesh Y. Dama

ABSTRACT

In recent years, remarkable technological advances have been made in particle chemistry performance, system optimization, detector design, and data processing and control. When brought together, the individual achievements in each discipline have created a step-function improvement in chromatographic performance ultra performance liquid chromatography (UPLC) is a modern technique which gives a new direction for liquid chromatography. UPLC which enhance mainly three areas of liquid chromatography: speed, resolution, and sensitivity. UPLC system column containing bridged ethylsiloxane / silica hybrid (BEH) structure with fine particle size (less than 1.7 m) is utilized and particle less than 2μm in diameter to acquire better resolution, speed, and sensitivity compared with HPLC. In twenty first centenary pharmaceutical industries are focusing for new ways to in economy and shorten time for development of drugs and drug product. The separation and quantification in UPLC is done under very high pressure (up to 100m pa). As contrast to HPLC, under high pressure it is observed that not any negative influence on analytical column and also other components like time and solvent consumption is less in UPLC. The commence review article is an endeavor to offer pervasive awareness around the development and Validation details about the UPLC and related techniques with the aim on practice to an estimation of medicinal Active agents in the last 10 years. The article also focused on general overview of UPLC. Method development and validation of UPLC method for estimation of drug and drug product.

Keywords: Drug, UPLC, HPLC, Resolution,