EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia ,

ISSN 2394-3211

Impact Factor: 7.065

ICV - 79.57

Abstract

NEW SIMPLE AND VALIDATED DENSITOMETRIC METHOD FOR DETERMINATION OF AMOXICILLIN AND CLOXACILLIN INTERACTION

Dr. Yahya Abduh Salim Mohamed*

ABSTRACT

Drug-drug interaction of amoxicillin and cloxacillin was studied by this method. The proposed method depends on measuring the optical density of the inhibited zone area using digital camera and Image J software. Image J software can measure the area even when the inhibition zone is irregular in shape thus avoiding measurement errors during diameter measurement by help of ruler. Good correlations were obtained between the area and the antibiotics concentrations in the range (0.990-0.992). The limits of detection and limits of quantitation were 44.82, 14.51 and 10.86 μgmL-1 and 135.82, 43.98 and 32.91 μgmL-1 for amoxicillin, cloxacillin and their binary mixture; respectively. The proposed method was validated according to US-Food and drug administration (FDA) guidance for bioanalytical method validation and USP 31 guidelines.

Keywords: Amoxicillin; Cloxacillin; Antibiotic activity; Drug drug Interactions; Minimum inhibitory concentration.

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