NEW SIMPLE AND VALIDATED DENSITOMETRIC METHOD FOR DETERMINATION OF AMOXICILLIN AND CLOXACILLIN INTERACTION

Dr. Yahya Abduh Salim Mohamed*

ABSTRACT

Drug-drug interaction of amoxicillin and cloxacillin was studied by this method. The proposed method depends on measuring the optical density of the inhibited zone area using digital camera and Image J software. Image J software can measure the area even when the inhibition zone is irregular in shape thus avoiding measurement errors during diameter measurement by help of ruler. Good correlations were obtained between the area and the antibiotics concentrations in the range (0.990-0.992). The limits of detection and limits of quantitation were 44.82, 14.51 and 10.86 μgmL-1 and 135.82, 43.98 and 32.91 μgmL-1 for amoxicillin, cloxacillin and their binary mixture; respectively. The proposed method was validated according to US-Food and drug administration (FDA) guidance for bioanalytical method validation and USP 31 guidelines.

Keywords: Amoxicillin; Cloxacillin; Antibiotic activity; Drug drug Interactions; Minimum inhibitory concentration.