RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF RELATED COMPOUNDS OF OCTREOTIDE ACETATE DEPOT SUSPENSION (MICROSPHERES)
Jerripothula Ganesh* and Bandi Veera Babu*
The analysis of Octreotide Acetate and related compounds Depot Suspension (Microspheres) By using RP-HPLC method using columm Waters X-Bridge C18 (250 X 4.6 )mm, 5μm with Flow rate 1.0 mL/min, Injection volume 50 μL, Wavelength 210 nm, Run time 70 minutes, Elution using Gradient programm, the Rt for Octreotide was found to be 28.23, related compunds like N- Acetyl Octreotide was found to be 24.32 Des Threoninol Octreotide was found to be 34.24. The suitable was developed for the indetification of the related compounds present in the Octreotide Acetate formulation.
Keywords: Octreotide Acetate, N-Acetyl Octreotide, Des Threoninol Octreotide, RP-HPLC, Microspheres, Depot Suspension.
[Full Text Article]