EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

HISTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF PLANTS WITH ANOMALOUS SECONDARY STRUCTURES

Dr. C. Sathya, M. Vinothini and Dr. M. N. Abubacker*

ABSTRACT

Four anomalous secondary structure plants were selected [Achyranthes aspera L. (Amaranthaceae), Bignonia suberosa Roxb. (Bignoniaceae), Boerhaavia diffusa L. (Nyctaginaceae) and Nyctanthes arbor-tristis (Oleaceae)] and secondary metabolites like alkaloids, ascorbic acids, polyphenols tannins, and terepenoids were identified using histochemical methods. Stem hand sections were treated with respective reagents and the colour indication proved the presence of respective compounds. Alkaloid and ascorbic acid were present in all the four plants in higher concentration. Nyctanthes arbor-tristis have shown the presence of Terpenoid and Tannin also in higher concentration. Tannin is present in moderate concentration in A. aspera, B. suberosa and B. diffusa. Terpenoid present in lower concentration in A. aspera, B. suberosa and B. diffusa. Polyphenol is present only in A. aspera and found to be absent in other three plants. Antimicrobial screening was conducted using ethanolic leaf extracts of these plants. Two ml of leaf extracts were introduced into 10 ml of Nutrient Agar Medium. Clinical strains of bacteria such as Bacillus subtilis, Klebsiella pneumoniae, Proteus vulgaris and Streptococcus pneumoniae were inoculated using streak plate method. A. aspera as well as B. diffusa leaf extract was effective for K. pneumoniae and S. pneumoniae as total inhibition of growth in the culture plants. B. suberosa ineffective to all the four bacteria, whereas N. arbor-tristis is effective for P. vulgaris bacteria as total inhibition of growth was noticed.

Keywords: Anomalous secondary structure, Antibacterial activity, Histochemical methods, Secondary metabolites.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing