EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

BACTERIAL CONDITIONED MEDIA: AN EFFECTIVE AGENT AGAINST MYCOBACTERIUM SMEGMATIS BIOFILM

Goutam Mukherjee, Rusha Roy and Alok Kumar Sil*

ABSTRACT

Biofilm plays a major role in the pathogenesis of Mycobacterium tuberculosis. Due to the presence of this polymeric matrix, anti-mycobacterial agents fail to exert their activity and thus Mycobacterium exhibits drug resistance phenotypes. Considering this, the current study investigated anti-biofilm activity of several conditioned media obtained after different bacterial culture using Mycobacterium smegmatis as model organism. For this purpose, logarithmically growing Mycobacterium smegmatis cells were treated with 11 different conditioned media prepared from different soil bacteria. Among these, five of the conditioned media exhibited considerable activity against biofilm. Thereafter, these five conditioned media were added to biofilm encapsulated mycobacterial cells to verify their ability to disperse preformed biofilm. The result showed that all these five conditioned media have ability to disperse preformed biofilm. Consistent with this, when any of these five conditioned media was added along with either isoniazid or rifampicin to biofilm encapsulated mycobacterial cells, cell viability was reduced drastically indicating the fact that these five conditioned media augment anti-mycobacterial activity of isoniazid and rifampicin. Thus the present study identified five bacterial strains, the conditioned medium of which exhibited anti-mycobacterial biofilm activity and augmented the activity of isoniazid and rifampicin against biofilm encapsulated cells. Thus, the current study provides agents that have the potential to be used in anti-mycobacterial therapy and may help in public health management.

Keywords: Tuberculosis, Mycobacterium, Biofilm, Conditioned medium.


[Full Text Article]

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  • New Issue Published

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )