IN VITRO STUDY OF AN ETHANOLIC EXTRACT OF ECLIPTA ALBA HASSK. FOR HEPG2 CELL LINE

Dr. Kulkarni D. V., Dr. Tathe Mangal Suresh* and Dr. Harke Sanjay Ningappa

ABSTRACT

For developing bridge way between Ayurveda and modern researches with advance technologies in Ayurveda we tried here an ethanolic extract of Eclipta alba against hepatic cancer. Eclipta alba has potent anti-cancer activity and therefore can be successfully used in cancer management both as mainline treatment and an adjuvant medication with minimum side effects. With the help of this paper the Eclipta alba which will prove to be beneficial for the researcher planning clinical trial and it can be used in the clinical practices for treating hepatic cancer in near future. Future research on such a topic would help to identify safe and effective anticancer drugs and will further the discovery of their mechanism of action. We have used an ethanolic extract of Eclipta alba Hassk. for phytochemical analysis, TLC, HPLC analysis to test active chemical components in it. All tests proved that an ethanolic extract of Eclipta alba Hassk. containing many active chemical components, therefore our experiment showed the positive results for an ethanolic extract of Eclipta alba Hassk. against hepatic cancer. The srb assay results were used to evaluate the anti-cancer activity of the extract. The effects of whole plant extract on cancer cell line were studied and evaluated. The percentage of cell growth and cell viability were calculated from tabulated result values of srb assay. The experiment revealed that the average percentage of growth inhibition was 79.33%. Cell viability srb assay also showed significant growth inhibition, at the same time statistical analysis of srb assay also proved significant results. The research performed here is very useful for setting up of different extract studies of Bhringraj for its anticancer activity.

Keywords: Eclipta alba Hassk., HepG2, ethanolic extract, (Sulforhodamine B) srb assay.