THE LEVEL OF MYELOPEROXIDASE (MPO) AND ANTI- PROTEINASE 3 IN PATIENTS WITH ENTAMOEBA HISTOLYTICA

*Hwaida SH. AL-Mahdawy, *Dr. Farhan Abood Risan and **Dr. Khalil I. Abd Mohammed

ABSTRACT

The study was carried out during the period from the beginning of (November/2013 - November/2014) for detection of Entamoeba histolytica in patients with age range from (3-60) year who attended to AL-Yarmouk teaching hospital and AL-Tifil central hospital. The diagnosis done by microscopic examination. A total of 200 suspected patient there was120 infected with the parasite diagnosed by the direct examination method, a blood sample was taken from each one, as well as (60) healthy controls were involved. The study included the measurement of Anti-neutrophil cytoplasmic antibodies c-ANCA (Anti PR3) and anti- myeloperoxidase p-ANCA (MPO) levels by Enzyme linked immuno sorbent assay (ELISA). The results indicated: The prevalence of Entamoeba histolytica by using microscopic examination was 145 (72.5%) in comparison to 120(82.75%) by using Triage (Micro parasite panel test). The level of Myeloperoxidase (MPO) and Anti- proteinase- 3increased significantly (P<0.05) in sera patients in comparison to healthy control, but there is no-significant (P>0.05) differences between the gender in both groups.

Keywords: Entamoeba histolytica, Anti-pr3, MPO.