EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

ISOLATION AND MOLECULAR CHARACTERIZATION OF LUMPY SKIN DISEASE VIRUS IN EGYPT DURING 2017- 2018

Ahmed Hodhod, Emad Elgendy, Mervat I. Abd El-Moniem and Madiha S. Ibrahim*

ABSTRACT

Lumpy skin disease is not associated with high mortalities, but the economic losses accompanying its eruption are higher in cattle trade. Lumpy skin disease virus is a member of genus Capripoxvirus (CaPV), family Poxviridae and is the cause of lumpy skin disease. In our study, we isolated and molecularly characterized Lumpy skin disease virus circulating in Egypt from April 2017 to September 2018. A total number of 295 samples including skin biopsies (243), whole blood samples (50) and tick groups (2) were tested by real-time PCR. The results showed that 91.3%, 76% and 100% of skin biopsies, whole blood and tick samples were positive, respectively. Thirty positive samples were isolated on embryonated chicken eggs chorioallantoic membranes. The results showed that 24 samples (80%) displayed characteristic pock lesions. The results were confirmed by conventional PCR and all 24 samples were confirmed as lumpy skin disease virus. Two samples were sequenced and phylogenetic analysis showed high similarity between the isolated lumpy skin disease virus and sheep and goat poxviruses. Furthermore, the tick samples and skin biopsies showed higher viral titration indicating their usefulness for viral detection in suspected cases. Moreover, the real-time PCR is one of the rapid diagnostic tools that can be used for viral detection in endemic areas with high specificity and sensitivity compared to other routinely used tools.

Keywords: Lumpy skin disease virus, real-time PCR, Isolation, CAM, conventional PCR, Sequencing.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )