EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

DETECTION OF CLOSTRIDIUM PERFRINGENS AND THEIR VIRULENCE TOXINS IN BROILER CHICKEN

Abdelrhman Salah Elsharkawy, Dalia Talat, Asmaa Sh. Elnaggar and Madiha Salah Ibrahim*

ABSTRACT

The present study was conducted to evaluate the prevalence of C. Perfringens in broiler chickens in El Behiera, Alexandria and Matrouh governorates. Samples (n= 400) were collected from intestine (n=200; 100 apparently healthy and 100 diseased birds), feed (n=100) and litter (n=100). The prevalence of C. Perfringens was 57, 88, 52 and 60% in apparently healthy birds, diseased birds, feed and litter, respectively. Isolates were confirmed by Matrix-assisted laser desorption/ionization (MALDI). Antimicrobial sensitivity of isolated C. perfringens showed sensitivity to Cefotaxime, Chloramphenicol, Bacitracin, Norfloxacin, Ciprofloxacin, Doxycycline, Amoxicillin + Clavulanic acid and Clindamycin. The isolates were resistant to Gentamycin, Erythromycin, Amoxicillin, Ampicillin and Neomycin. Eighteen C. perfringens isolates from different samples were examined by PCR for Alpha, Beta, Epsilon and Iota genes. The alpha gene was detected in 17 of the examined isolates. Only five isolates were positive for the Tpel toxin gene. Further, the Net-B toxin gene was detected in one isolate. Environmental factors (feed & litter) in poultry farms represent an important source of C. perfringens infection (toxigenic types) and apparently healthy birds may act as a major source of infection. Alpha toxin was the predominant major toxin in our investigation and the Tpel gene was detected more than the Net-B gene.

Keywords: Broiler chickens, C. Perfringens, Tpel gene, PCR.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )