HUMAN BONE MARROW DERIVED STEM CELL MIGRATION AND PROLIFERATION UPON PLATELET RICH FIBRIN CONDITION
My NT Nguyen*, Hoang M. Lam, Ha LB Tran and Thuy AV Pham
ABSTRACT
Background: Periodontal diseases cause destruction of periodontal tissues and ultimately resulting into tooth loss. Various approaches including including guided tissue regeneration and bone grafts have been employed to accelerate either soft or hard tissue in periodontal repair. Platelet rich fibrin (PRF) is obtained by centrifuging peripheral wholeblood without anticoagulants. PRF has been used in clinical practice as a bioactive material in facilitating tissue regeneration. Aim of study: The study aimed to obtain and evaluate PRF effects on human bone marrow stem cells. Materials & Methods: PRF was prepared by centrifuging wholeblood without anticoagulants at 2500 rpm for 15 min. Liquid extract of PRF was obtained and evaluation for growth factors secretion by ELISA, including PDGF and TGF-1. hBMSC proliferation and migration upon PRF condition were assessed by MTT assay and scratch wound healing assay, respectively. Results: PRF clots were formed after centrufugation, which exhibited the release of PDGF and TGF-1 for up to three days of investigation. PRF liquid showed great biocompatibility to hBMSCs. Furthermore, PRF liquid significantly promoted the proliferation and migration in vitro. Conclusion: These results confirmed that PRF is completely suitable to be used as bioactive material for periodontal regeneration.
Keywords: Platelet rich fibrin, bone marrow derived stem cells, proliferation, migration, periodontal regeneration.
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