HIGH PRESSURE THIN LAYER CHROMATOGRAPHY (HPTLC) FINGERPRINT PROFILE OF EUGENOL FROM PIPER BETEL LEAF EXTRACT
Dr. Anjum Aara*, Dr. Vani Chappidi and Dr. Madhavan Nirmal Ramadas
The present study was aimed to identify and isolate Eugenol in the methonolic extract of Piper betel leaf. HPTLC fingerprinting was carried out for Eugenol in methonolic extract of Piper betel leaf. For confirming the presence of Eugenol in Piper betel leaf extract, the available standard Eugenol was taken as marker. Presence of Eugenol was checked both at 254nm and 366nm. At 366nm HPTLC chromatogram showed presence of Eugenol in the Piper betel leaf extract as bluish coloured band (T4), (T5), (T6) at concentrations 4,6, 8 μg/ml. HPTLC fingerprinting of both Piper betel leaf extract and the standard (Eugenol) was carried out at concentrations 4,6,8 μg/ml. In all the three concentration levels of the Piper betel leaf extract, presence of Eugenol was evident.
Keywords: High Pressure Thin Layer Chromatography (HPTLC), Fingerprint Profile, Methanolic Extract and Piper Betel Leaf.
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