DEVELOPMENT OF VALIDATED ANALYTICAL METHOD FOR FORMOTEROL IN PURE AND DOSAGE FORM BY USING RP-HPLC

Vuyyuri Kasi Viswanath* and Ch. M. M. Prasda Rao

ABSTRACT

A Simple, precise, accurate method was developed by simultaneous estimation of Formoterol in dosage form, an easy, accurate, linear technique was optimized. Chromatogram runs through BDS C8 150 x 4.6 mm, 5μm.Mobile phase comprising Buffer 0.1 % OPA: Acetonitrile taken in the 45:55 ratios was pumped through the column at a flow rate of 0.8 ml / min. The selected optimized wavelength was 223 nm. Formoterol retention time were obtained to be 2.2 min the %of RSD of Formoterol was 0.4, The % recovery for Formoterol was 99.10 % alone. LOD, LOQ values acquired from Formoterol were 0.03, 0.10. Formoterol regression equation is y = 83345x + 10897 and passes the regression coefficient 0.999. Retention time, runtime was reduced, so the technique created has been easy and economical that can be used in periodic quality control tests in industries.

Keywords: Formoterol, Acetonitrile, O-Phosphoric acid, RP-HPLC.