A PARTIALLY PURIFIED LIPOPOLYSACCHARIDE EXTRACTED FROM KLEBSIELLA PNEUMONIA AS ANTIBACTERIAL
Ruaa SH.*, Suhad Mohammad and Esraa Sameer
50 isolates of Klebsiella pneumonia were isolated from150 urine specimens taken from recurrent –urinary tract infections (UTI) patients attended to AL-Sadar Hospital. Specimens were cultured on specific media, and then bacterial isolates were identified depending on morphological, biochemical and VITK-2. The results showed that Klebsiella pneumonia comprise 29 (58%), 5 (10%) and 16 (32%) from recurrent UTI, kidney stone and Catheters samples respectively. The isolate that appeared multidrug resistance(MDR) to most antibiotics used in this study were chosen to extracted lipopolysaccharide. The results of The antibacterial activity assay of LPS extract of Klebsiella pneumonia were done against E. coli, Klebsiella pneumonia, Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Bacillus subtilis, Staph aureus, and Streptococcus pneumonia at different concentration (200,300,400) μg /ml were evaluated by measuring the diameters of zones of growth inhibition on bacterial, and compare with the inhibition zone of amoxicillin as control, the result show all bacteria sensitive to LPS (100%) at concentration (200,300,400) μg /ml and the inhibition zone ranging from (25 to33)mm, compare with amoxicillin that all bacteria resistance to it(100%). In Conclusion: LPS are promising a new medicine lead to kill gram positive and gram-negative bacteria.
Keywords: Lipopolysaccharide, Klebsiella pneumonia, urinary tract infection, amoxicillin, Antibiotics.
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