QUANTITATIVE ESTIMATION OF CAFFEINE IN SOFT DRINKS USING UV-VISIBLE SPECTROPHOTOMETRIC METHOD

Y. Ratna Sindhu*, P. Lavanya, P. Pushpa Divya, P. Udaya Swetha Sri and K. Venkata Divya

ABSTRACT

Caffeine is a well-known stimulant which is added as an ingredient to various carbonated soft drinks. Caffeine has drawn more attention due to its physiological effects beyond that of its stimulatory effect. Consumers are interested in knowing the exact amounts of caffeine existing in beverages. It is important to obtain information about the drinks because they are widely consumed all over the world. Various analytical techniques are used for tea analysis like HPLC because these techniques have high accuracy and precision but UV-Vis spectrophotometry is mostly used because it is cheap and easily available in laboratories. Results showed the largest amount of caffeine was present in Brand X and the smallest amount - Brand-Z. as per the US FDA the daily intake of the caffeine content not more than the 200-ppm concentration.

Keywords: Caffeine, accuracy, precision.