EVALUATION OF ALPHA-AMYLASE AND ALPHA-GLUCOSIDAE INHIBITORY POTENTIAL OF SEDUM SARMENTOSUM

In Sook Kye and Man Kyu Huh*

ABSTRACT

The purpose of present study was to investigate the effects of extracts of Sedum sarmentosum a traditional vegetative plant, on α-amylase and α-glucosidase activities in vitro. The three tissues (leaves, stems, and roots) of S. sarmentosum were extracted with distilled water (AE) and aqueous methanol (MeOH-H2O) were obtained from MeOH-H2O crude extracts per liquid/liquid extraction. The results of the both enzyme inhibition activity was found in a dose-dependent manner. The high α-amylase inhibitory found on leaves extracts. α-amylase inhibition effects of leaves of S. sarmentosumevaluated at 1.0 μg/ml of distilled water extract (DWE) was 29.4% and that of stems was 25.1% at same concentration. The values of α-amylase IC50 for leaves with DWE and ETE were 15.11 μg/ml and 16.08 μg/ml, respectively. Those of α-glucosidase IC50 for leaves with DWE and ETE were 14.55 μg/ml and 13.64 μg/ml, respectively. α-glucosidase inhibition for DWE of leaves evaluated at 1.0 μg/ml was 50.5% and that of stems was 39.7% at same concentration. All extract from this plant possess moderate α-amylase inhibition with potent α-glucosidase inhibitory activity.

Keywords: ?-amylase, ?-glucosidase, Sedum sarmentosum.