EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

TETRA-PRIMER ARMS-PCR AS AN EFFICIENT ALTERNATIVE FOR SNPS DETECTION IN MOLECULAR DIAGNOSTIC: A COMPARISON STUDY

Sabah Linjawi*, PhD, Zuhoor Al-Gaithy, MBBCh, FRCS (I), Samar Sindi, MS, Norah Hamdi, MS, Ayman Linjawi, MD, SRCS (CA) and Aisha Alrofidi, PhD.

 

ABSTRACT

Objective: The aim of this research was to compare the efficiency of the tetra-primer amplification refractory mutation system–polymerase chain reaction (ARMS-PCR) with restriction enzyme for detection of SNP anomalies in breast cancer blood samples. Method: one hundred breast cancer patients were analyzed for SNP mutation using tetra-primer ARMS-PCR technique and restriction enzyme (Pst1), to identify allele distribution of LCN2 on exon one a SNP rs11556770 (G/T). In this case, a single nucleotide transforms the amino acid from Glutamine, Gln (CAG), to Histidine, His (CAT). Result: Both techniques, tetra-primer amplification refractory mutation system–polymerase chain reaction (ARMS–PCR) and restriction enzyme, yielded normal (non-pathogenic) genotype pattern for the same SNP of sets samples, wherein tetra-primer ARMS PCR was 444bp, 156b, While restriction enzyme (PstI) three band 261bp, 135bp, 48bp. Nevertheless, both results estimate a normal allele for both techniques. Conclusion: Tetra-primer arms PCR method attained high specificity and sensitivity, along with the fact that this technique is more affordable, it can therefore be concluded that this method is an efficient and convenient option for detection of SNP anomalies for diagnostic purposes.

Keywords: T-ARMS-PCR, breast cancer, LCN2, Pst1, SNP.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia