SIMULTANEOUS ESTIMATION OF ATENOLOL AND CHLORTHALIDONE IN COMBINE TABLET DOSAGE FORM BY DUAL WAVELENGTH METHOD USING UV-VIS SPECTROPHOTOMETRY

Welankiwar AS* and Charde MS

ABSTRACT

A simple, precise, reproducible, accurate, economical & rapid UV-VIS Spectrophotometric method have been developed and validated for the simultaneous estimation of ATN and CTN in tablet dosage form. This paper describes the dual wavelength method as a quantification parameter. In this method the two wavelengths are selected for each drug in way that the differences in absorbances are zero for another drug. ATN has equal absorbance at 232.0 & 218.0 nm which are used for the quantification of CTN while CTN has equal absorbance at 260.0 nm & 242. 0 nm which are used for the quantification of ATN. The developed method obeys the beers law in the concentration range of 40-80μg/mL for ATN and 10-50 μg/mL for CTN. The recovery studies shows %RSD for ATN 0.21 and for CTN 1.34 by this method. The results of analysis have been validated statistically for accuracy, precision, repeatability, specificity and ruggedness. The method was successfully applied to the determination of these drugs in pharmaceutical dosage form.

Keywords: ATN, CTN, UV-VIS, Spectrophotometry, Dual Wavelength, Assay Method.