EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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 ICV - 79.57

Abstract

IN-VITRO PROPAGATION OF ALPINIA GALANGA L. VIA SOMTIC EMBRYOGENESIS – A MEDICINAL PLANT

Dr. Mustafaanand P.H.

 

ABSTRACT

Unscientific collection of medicinal plants from the wild nature became many medicinal plants in endangered and threatened condition. Many members of the Zingiberaceae have became Vulnerable and threatened. Vegetative propagation of Alpinia galanga Linn (Zingiberaceae) cannot fulfill the current demand for planting material and so tissue culture offers an alternative means for mass propagation. Propagation of A. galanga via colonal propagation and organogenesis were successfully standardized and reported earlier by the same author. Propagation by somatic embryogenesis is not reported yet. This paper deals with propagation of A. galanga via somatic embryogenesis. Rhizome discs were inoculated in M.S. medium supplemented with various concentrations of BAP, KIN and 2, 4-D individually or in various combinations. Rhizome discs were produced embryogenic callus when it cultured in M.S. medium supplemented with 2Mg/L. BAP or KIN and 0.25 Mg/L.2,4-D. This embrogenic callus was transferred into a medium having higher concentrations of BAP or KIN + 2, 4-D (3Mg/L + 0. 25Mg/L), produced an average of 4.0 well developed globular embryos. On latter cultures, the auxin were completely removed, well rooted plantlets were emerged from the cultures. Fully developed plantlets were acclimatized successfully in a mixture of soil, sand and compost (1: 1: 1). Survival of the established plantlets was 40%. This method can be used for the large scale production planting material, and germplasm conservation of this threatened medicinal plant. Moreover, the callus can be further utilized for suspension cultures for secondary metabolite production.

Keywords: Alpinia galanga, Embryogenic callus, Somatic embryo genesis, 6-Benzylaminopurine (BAP), Kinetin (KIN), 2, 4- Dichlorophenoxyacetic acid.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia