METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF PANTOPRAZOLE, DICLOFENAC & CHLORZOXAZONE BY RP-HPLC METHOD

Geddada Bhavana*, Dr. Devanaboyina Narendra, Gadi Vijaya Lakshmi and Rajanala Rajeswari

ABSTRACT

A simple, accurate, precise method was developed for the simultaneous estimation of the Chlorzoxazone (CLZ), Pantoprazole (PTP) and Diclofenac (DCF) in solid dosage form. Chromatogram was run through Discovery C18 150x4.6mm, 5. Mobile phase containing Buffer and Acetonitrile in the ratio of 42:58 v/v was pumped through column at a flow rate of 0.75ml/min. Buffer used in this method was 0.1% Orthophosphoric acid (OPA). Temperature was maintained at 30°C. Optimized wavelength for Chlorzoxazone. Pantoprazole and Diclofenac was 229.0 nm. Retention time of Chlorzoxazone, Pantoprazole and Diclofenac were found to be 2.233min, 2.763min and 3.759min %RSD of system precision for Chlorzoxazone, Pantoprazole and Diclofenac. were and found to be 1.0, 0.9and 0.6 respectively. %RSD of method precision for Chlorzoxazone, Pantoprazole and Diclofenac.were and found to be 0.6, 0.2 and 0.3 respectively. % recovery was Obtained as 99.88%, 99.81% and 99.75% for Chlorzoxazone, Pantoprazole and Diclofenac. respectively. LOD, LOQ values are obtained from regression equations of Chlorzoxazone, Pantoprazole and Diclofenac. were 0.14ppm, 0.22ppm, 2.88ppm and 0.42pm, 0.67ppm ,8.72 ppm respectively. Regression equation of Chlorzoxazone.wasy= 6226.1x + 745.53, Pantoprazole was y = 17987x + 3754.7. and of Diclofenac was y = 10072x + 32901.

Keywords: Chlorzoxazone, Pantoprazole, Diclofenac, RP-HPLC.