CROTALARIA LABURNIFOLIA POLLEN – BIOCHEMICAL ANALYSIS

*Sandhya Deepika D., Laxmi Sowmya K., and P. K. Ratna Kumar

ABSTRACT

A pollen grain is a microscopic body that contains the male reproductive cell of plant. It is crucial in plant's fertilization process. Good fruit set and high crop yield depend to a great extent on healthy and viable pollen grains. The present paper deals with analysis of carbohydrates, DNA, RNA, free amino acids and total protein content in pollen of the Crotalaria laburnifolia. The biochemical evaluation of these metabolites was performed by standard methods. Estimation of carbohydrates was done by Anthrone method. The estimation of DNA and RNA were done by using UV Spectrophotometer. Amino acids estimation was done by Ninhydrin method and separation and identification by thin layer chromatographic method and estimation of protein was done by Lowry’s method. Amino acids like Leucine, Phenylalanine, Methionine, Aspartic acid, Histidine, Tryptophan were found to be present.

Keywords: Biochemical evaluation, amino acids, protein, Carbohydrates, DNA, RNA, Crotalaria laburnifolia.