VERAPAMIL - HYDROCHLORIDE AT LOW CONCENTRATIONS INHIBITS THE GROWTH OF RELAPSE GLIOBLASTOMA

N. Ya. Gridina* A. N. Morozov, V. D. Rozumenko, A. A. Shmeleva and Yu. V. Ushenin

ABSTRACT

The life expectancy of glioblastoma patients was investigated in the distant postoperative period. These patients were treated with such concentrations of verapamil – hydrochloride, that minimize the level of blood cells aggregation in II stage of the inflammatory process. 11 patients received treatment (I group) and 32 patients did not received treatment (II group) with calcium blocker using verapamil – hydrochloride examples in pills. For the objectification of tumor association inflammation (TAI), that present’s in the patient’s body, the definition of blood cells aggregation was examined on the “Plasmon” sensor. Peripheral blood cells were collected from patients to determine the indicators of blood cells aggregation with the addition of various aqueous of verapamil – hydrochloride (from 1: 10 to 1: 100,000 times). Patients took drugs at the lowest level of blood cells aggregation doses. Drug dose was performed by decreasing its concentration by a factor of 10, 000. From the 43 patients, admitted to the clinic, only 3 patients did not undergo chemotherapy at will. After the surgical removal of glioblastoma and the postoperative irradiation course, they only took verapamil – hydrochloride at low concentration daily. In the I group of 8 patients, undergoing combined treatment courses and taking verapamil - hydrochloride, the average life expectancy was 18.6 ± 1.82 months and 8.47 ± 1.02 months in the group of 32 patients (Cox’s F - test p = 0,00108). Three patients without chemotherapy in the postoperative period received daily treatment with verapamil – hydrochloride without interruption for life. All 3 patients continue to live for 19, 25 and 29 months, respectively. The paper presents for the first time the results of highly malignant glioblastoma treatment with low concentrations of the NMDA-dependent calcium blocker verapamil – hydrochloride. The results indicate a high antitumor activity of the drug, both when used together with traditional methods of treatment or separately.

Keywords: Glioblastoma, tumor-associated inflammation, verapamil-hydrochloride, low concentrations, life expectancy.