EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

EVALUATION OF CELL DEATH MECHANISMS INDUCED BY HYPERICIN AND ITS DERIVATIVE IN COLORECTAL CARCINOMA MODELS

Youssef M. Shehata, Hala M. Ali* and Nermin Raafat

ABSTRACT

Colorectal cancer (CRC) is the third most malignancy and the fourth human killer. Photodynamic therapy (PDT) has emerged as a promising alternative to conventional cancer therapies. This study aimed to investigate the ability of Hypericin (HY) and its derivative (HAHY) as synthetic photosensitizers (PSs) to induce tumoricidal effect in HCT-116 carcinoma models. Sixty balb/c male mice were transplanted with 5×105 HCT-116 cells to induce carcinoma bearing mice model. The experimental animals were then divided into five groups; Group I (untreated), Group II (HY-PDT), Group III (HAHY-PDT), Group IV (HY-PDT+EGCG) and Group V (FOLFOX). Blood, liver and tumor mass samples were taken. Also, biochemical studies and MTT assay were performed. HY and HAHY - PDT had a significant cytotoxic effect on human HCT-116 cells. Also, a significant upregulation in gene expression of P53 and CYT-c in concentration dose dependent manner. Furthermore, a significant increase in IL-6 gene expression in group (2) higher than that in group (3).Whereas it decreased in group (5), and showed insignificant difference in group (4) .Also, results showed decrease in VEGF gene expression in both group (3) and (4), while it showed significant increase in group (2) and slight increase in group (5). In conclusion, these PSs possess a strong cytotoxic and apoptotic effect on human HCT-116 cells. Using of EGCG along with HY-PDT improves the outcome. Also, HAHY-PDT can be used as an alternative to HY-PDT, where it showed tumor shutdown without metastasis besides its antitumor immunity.

Keywords: Hypericin; Hexa Acetyl Hypericin; Colorectal cancer; Photodynamic therapy; Synthetic photosensitizers.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )