HPTLC ANALYSIS OF GLYCOSIDES PROFILE IN THE LEAF AND BARK SAMPLES OF LORANTHUS LONGIFLORUS DESR COLLECTED FROM TWO HOST TREES.

L. Chandrakasan and R. Neelamegam*

ABSTRACT

HPTLC determinations of glycoside compound profile was carried out in the methanolic extract of leaf and bark samples of Loranthus longiflorus collected from two host trees –Casuarina equisetifolia and Ficus religiosa, and compared. The methanol extract of L. longiflorus leaf samples obtained from C. equisetifolia and F. religiosa host trees showed 7 and 6 compounds, respectively, and were compared with stevioside standard. Among the compounds, 5 and 4 compounds in each sample, respectively, was identified as glycosides while other compounds were unknown. Three compounds from each L. longiflorus leaf samples collected from C. equisetifolia (peak no. 2, 6 & 7) and F. religiosa (peak no. 1, 3 & 4) host trees showed similar Rf values (0.12, 0.80 & 0.86, respectively). On the other hand, the methanol extract of L. longiflorus bark sample collected from C. equisetifolia and F. religiosa host trees contained 17 and 14 compounds in each sample, respectively and were compared with stevioside standard. Among the compounds, 6 compounds, in each sample, was identified as glycosides while other compounds were unknown and 3 compounds from both bark samples obtained from C. equisetifolia (peak no. 4, 9 & 14) and F. religiosa (peak no. 4, 9, & 14) showed similar Rf values (0.22, 0.59 & 0.77). None of the compounds from leaf and park samples of L. longiflorus collected from C. equisetifolia and F. religiosa showed similar Rf values. The results of present study indicate that the HPTLC analysis of methanol extracts of L. longiflorus leaf and bark samples from C. equisetifolia and F. religiosa host trees make certain the presence of glycosides and the host trees influenced on the nature and number of glycosides present in the hemiparasitic plants.

Keywords: Hemiparasite, Loranthus longiflorus, Leaf sample, bark sample, methanol extracts, Casuarina equisetifolia host, Ficus religiosa host, HPTLC analysis, Glycoside profile.