ANTI-INFLAMMATORY EFFECTS OF HIPPOPHAE RHAMNOIDES AND BOSWELLIA EXTRACTS ON MASSETER MUSCLE AND GASTROCNEMIUS INFLAMMATION IN RATS

Hae-Jin Jeong, Byeong-Jo Kim and Tae-Heon Kim*

ABSTRACT

This study aimed to establish an inflammatory model by injecting carrageenan into masseter muscle and gastrocnemius muscle in rats, and then verify the anti-inflammatory effects of both Hippophae rhamnoides and Boswellia extracts. As experimental animals, male Sprague-Dawley rats aged 7-8 weeks (250-300 g) were used. The inflammation of masseter muscle and gastrocnemius was induced by injecting 0.3 mL of 2% carrageenan diluted with normal saline solution into them. Hippophae rhamnoides and Boswellia extracts were orally administered into the rats, 30 minutes before the inflammatory induction. In order to verify changes in tissues, masseter muscle and gastrocnemius muscle were extracted, 4 hours after the injection of the carrageenan. The tissues were photographed by using an optical microscope, after slicing and staining of them, and the area of cells was measured by using Image J (1.52a, USA). For the quantitative evaluation of measured cell changes, SPSS statistic ver. 22 was used to conduct a statistical analysis. The results of this experiment show the significant dose-dependent increase in the area of cells of most rats in the extract administration group, compared to the carrageenan group. The sizes of muscle cells in masseter muscle, especially Bos30 group and Bos15+Vit150 group, were significantly increased, depending on the concentrations of both vitamin tree and Boswellia extracts. For the gastrocnemius muscle, anti-inflammatory effects were induced in the extract administration group, compared to the control group, but the effects were not significant. From the results, the effects of Hippophae rhamnoides and Boswellia on the inflammatory regulation of masseter muscle and gastrocnemius could be proved, suggesting that natural substances can be effective drug for the inflammatory regulation of muscle.

Keywords: Hippophae rhamnoides, Boswellia, Inflammation, Masseter Muscle, Gastrocnemius.