EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

PROTEIN FRACTION FROM ACTINOMYCETES ISOLATES INHIBITS PROTEASE ACTIVITY SECRETED BY STAPHYLOCOCCUS AUREUS

Ashwini B. and Manjula I. K.*

ABSTRACT

The pathogenic potential of staphylococcus aureus is attributable by the production and secretion of various toxins, pathogenic factors such as various enzymes that include proteases which collectively contribute to the virulence of the organism. The present study focused on targeting proteases that were secreted into the growth medium during the growth of Staphylococcus aureus. The proteases that were secreted by the Staphylococcus aureus were checked for their inhibition activity by the cell free culture extracts from actinomycetes isolates that were isolated from maize-rhizosphere soil. The isolation of actinomycetes from maize-rhizosphere soil was done by serial dilution plating and cellulose ester membrane isolation methods. Five isolates belonging to Streptomyces sp, Microbispora sp, and Thermomonospora sp. exhibited protease inhibition as assayed by well diffusion activity using skim milk agar media and observation of the extent of hydrolysis zone due to protease activity. The protein fractions with protease inhibition activity were extracted from the actinomycetes isolates by protein purification that resulted in the separation of prominent protein bands after subjecting to electrophoretic separation technique.

Keywords: Staphylococcus aureus, Proteases, Actinomycetes isolates, Protease inhibition.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia