HISTOLOGICAL STUDY OF EXTRACTION OF LIPOPEPTIDE BIOSURFACTANT FROM BACILLUS SUBTILIS IN OIL SPILLED SOIL ON WHITE RATS TISSUE
Wafaa H. AL-Hashemi* and Ghofran F. AL-Jubuo
Thirty bacterial isolates were obtained from 20 oil contaminated soil samples from various automobile work shops, cars fuel stations, of three different areas in Al-Najaf city. About 18 isolate suspected to belong to genus Bacillus. All these isolates were subjected to microscopic examination and cultural characteristics, about 12 isolate belonged to genus Bacillus subtilis. 2 isolate were selected for the purpose of knowing their ability to produce biosurfactant. All these isolates were screened for their ability for biosurfactant production through blood haemolysis activity on blood agar indicated that all isolates were β -haemolysis, the safety of the extract was studied on white rats tissues including liver, kidney, spleen and intestine. The results showed no damage to tissue compared with control treatment.
Keywords: Bacillus subtilis, Biosurfactant, Albino Rats.
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