A HIGH PRECISION PHYTOCHEMICAL STUDIES OF ANNONA MURICATA L. BY LCMS STUDIES

John Thomas,* Jisha James, Nekha Raj, Shabana Sharoff and Niveditha Dileep

ABSTRACT

Phytochemical studies helps to reveal the chemical nature of secondary metabolites which is pharmacologically effective for many of the ailments. Conventional methods are much time consuming and be less efficient to reveal the chemistry of the phytochemical constituents. High performance liquid chromatography coupled with mass spectrometry (LC/MS) can be an efficient tool for the accurate and efficient phytochemical studies. Annona muricata are traditionally used to treat a variety of ailments including inflammations, bacterial infections, herpes virus infections, and cancer. The alcoholic extract of leaves was subjected to phytochemical evaluation by high performance liquid chromatography coupled with mass spectrometry method (LC/MS) for the studies of various phytoconstituents. The LC-MS Analysis revealed 29 constituents of which 17 of the compounds were identified and matched to the Metwin2.0 library data. The current study suggests that ethanol extract of leaves of Annona muricata have revealed the presence of many phytochemicals that assures the therapeutic efficacy of the Annona muricata.

Keywords: Annona muricata; Ethanolic extracts; LC-MS; Phytochemicals.