SIMULTANEOUS UV-VISIBLE SPECTROPHOTOMETRIC DETERMINATION OF ETOPOSIDE AND PIPERINE ANALOG-I IN BULK AND PHARMACEUTICAL FORMULATION BY FIRST-ORDER DERIVATIVE SPECTROSCOPIC METHOD
Sachin Bhusari*, Gayatri Borse and Pravin Wakte
A simple, rapid, accurate and precise method was developed for the simultaneous estimation of Etoposide and Piperine Analog-I in bulk and pharmaceutical formulation using first-order derivative spectrophotometric method. The zero-order spectra were obtained over the range of 200-400 nm for different concentration of Etoposide and Piperine Analog-I, the overlay was converted to the first-order derivative spectra and zero-crossing points were selected as working wavelengths. Multiple calibration standards of both the drugs were prepared separately and absorbance values were recorded at respective wavelengths. The proposed analytical method was validated according to ICH guidelines and various parameters viz. accuracy, precision, Limit of Detection, Limit of Quantitation, ruggedness, and robustness were assessed. The zero-crossing point of Etoposide and Piperine Analog-I were found to be 360 nm and 250 nm respectively. The linearity range was found to be between 10-60 μg/ml for Etoposide and 2-10 μg/ml for Piperine Analog-I with correlation coefficient of 0.999. The intra- and inter-day precision study showed percent relative standard deviation between 0.19-1.34 and 0.25-1.03 respectively. LOD and LOQ of proposed method were found to be 0.1195 μg/ml and 0.3623 μg/ml for Etoposide respectively whereas for Piperine Analog-I, said values were 0.0372 μg/ml and 0.1129 μg/ml respectively. The total percent recovery of Etoposide and Piperine Analog-I was found to be in the range of 99.83 to 101.02 and 99.81 to 100.87 respectively.
Keywords: UV-visible spectrophotometric method, first-order derivative spectrophotometric method, Etoposide and Piperine Analog-I.
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