SYNTHESIS AND CANCER INHIBITION OF POLYAMINES FROM REACTION OF 3- AMINO-1,2,4-TRIAZOLE (3-AT) AND GROUP 4 METALLOCENE DIHALIDES

Charles E. Carraher, Jr.*, Michael R. Roner, Ryan Crichton, Jessica Frank, Alisa Moric-Johnson, Lindsey C. Miller, Francesca Mosca, Paul Slawek

ABSTRACT

The formation of polyamines from reaction of 3-amino-1,2,4-triazole, 3-AT, with Group 4 metallocene dichlorides occurs rapidly employing the interfacial polycondensation reaction giving moderate yield and chain lengths. MALDI MS produces ion fragments to six to nine units with good isotopic abundance matches. IR shows the absence of the internal ring NH consistent with the formation of M-N bands and inclusion of bands from both the metallocene and 3-AT units. NMR results are also consistent with the proposed repeat unit. The polymers show good inhibition of a group of solid human cancer cell lines including breast, lung, prostrate, and pancreatic cancer and glioblastomas brain cancer cell lines. Thus, it is a wide-ranging potential anticancer drug.

Keywords: 3-amino-1,2,4-triazole, 3-AT, group 4 metallocenes, brain cancer, breast cancer, pancreatic cancer, polyamines, group 4 metallocene polymers, interfacial polymerization, glioblastomas brain cancer.