EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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Abstract

PHYTOCHEMICAL STUDY AND ANTIOXIDANT ACTIVITY OF EXTRACT FROM THE LEAVES OF LEBANESE DATURA METEL L

Ali Jaber*, Lamis Al-Harakeh, Ghassan Ibrahim and Edmond Cheble

ABSTRACT

Leaf extracts of Datura metel, obtained from south Lebanon, were tested for phytochemical and antioxidant activities. The phytochemical screening of extract revealed the presence of some alkaloids, flavonoids, saponins, tannins and steroids. Quantitative determination of Essential Oils content was conducted by hydrodistillation, while total alkaloids are determined by gravimetric method. Whereas total phenolics, total flavonoids, and in vitro antioxidant activities (DPPH and reducing power assay) of methanolic extract were carried out using colorimetric methods. The total phenolic content, expressed as mg of gallic acid equivalent (GAE) per gram of dry weight, was found to be 5.97 ± 0.7 mg GAE/g. The radical scavenging activity measurement, expressed in terms of IC50, was found to be 400.36 ± 9.07 μg.mL-1. These extracts also showed marked reducing power using the potassium ferricyanide reduction method. Consequently, the Datura metel plants studied would be considered as promising sources of bioactive phytochemicals. Further investigations are a must to evaluate other biological activities of this plant.

Keywords: Datura metel L., Total alkaloids, Total phenolics, Total flavonoids, Antioxidant activity.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia