EFFICIENCY OF MECHANICAL FORCE IN ESTABLISHMENT OF ASHERMAN'S SYNDROME MOUSE MODELS

Tuyet Thi Vi Le, Hoang Minh Lam and Ha Le Bao Tran*

ABSTRACT

Asherman's syndrome also called intrauterine adhesions, is the formation of scar tissue after uterine surgery that can cause infertility. The establishment of Asherman's syndrome on animal models is necessary to develop therapies for the treatment of this syndrome. This study aimed to create Asherman's syndrome mouse model by mechanical force. Using 25G needles, we demonstrated that the injuries created by mechanical force are capable of forming scars into the uterus after four days by histological evaluation. In addition, there was a significant difference in numbers of endometrial glands between the injured group (15.33 ± 1.23) and the control group (33.00 ± 4.58) at day 0 (P-value < 0.05). The numbers of endometrial glands of each group were also unchanged after three estrous cycles (P-value > 0.05). Furthermore, we also demonstrated that the injured uterus was an inability to conceive after Asherman's syndrome mice mated. In summary, the effects of needle lead to endometrial injuries, intrauterine adhesion formations, and failed implants. The established mouse models are materials for Asherman's syndrome therapeutic researches.

Keywords: Asherman's syndrome, endometrial injury, estrous cycle, mechanical force, mouse model.