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An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

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European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57



Rajendra Phadke*, Ganesh Hande, Deepesh Patil and Dr. Amit Gosar


The purpose of this research study is to develop simple, precise, accurate and economical method for determination and speration of Aminophenol isomers by reserverse phase chromatography. This chromatographic method was developed on Thermo, Hypersil BDS C18, 250 mm x 4.6 mm, 5 μm or equivalent column. The mobile phase used for speperation was 0.01M diammonium hydrogen phosphate buffer adjusted pH 5.5±0.05 with ortho phosphoric acid with secondary mobile phase is methanol. The flow rate of mobile phase was 1.0 ml per minute with intial composition 90 % diammonium hydrogen phosphate buffer and 10% methanol solvent changing to 30% and 70% within 15 minutes then stabalised with initial composition of mobile phase.The detection of isomer of aminophenol was observed at walength 272 nm and peak responcese are very good. The deteceted isomer is about 0.003ppm and further to be quitified at 0.01ppm level concentration. The relative standard deviation of isomer aminophenol was 0.69%. This developed method was validated as per ICH guideline and found out to be linear, accurate, specific, selective, precise, and robust. The correlation coefficient for main aminophenol was 1.000 and the co-isomer re-sponceses where linear from 0.10 ppm to 0.8 ppm. The obtained recovery of isomers were found to be between 95 to 105 percentage and the sample solution was stable up to 12 hrs in dark places hence this method can be success-fully applied for the determination of isomers of Aminophenols.

Keywords: Aminophenol, Isomer, Binary Gradient, HPLC, method development, validation.

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia