EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

THE ROLE OF COAGGREGATION INHIBITORS IN ENHANCING THE ANTIBACTERIAL EFFECT OF IRRIGANTS INSIDE THE ROOT CANAL- AN IN VITRO STUDY USING CONFOCAL AND MOLECULAR PROBE ASSAY

*Dr. Hannah Rosaline, MDS, Dr. Deivanayagam Kandswamy, BDS, MDS, FDS, Dr. J. Kalyani, MBBS, DGO, MD, Dr. Velayutham Gopi Krishna, BDS, MDS, PhD and Dr. Chinambedu D. Priya, M.Sc, PhD

ABSTRACT

Introduction: Root canal infections are polymicrobial. Coaggregation between bacteria in the root canal play a potential role in endodontic infection. The aim of this study was to investigate the antibacterial efficacy of 3% sodium hypochlorite and 2% chlorhexidine pretreated with coaggregation inhibitors against dual species biofilm of E.faecalis and F.nucleatum. Methods: Dentine samples from human uniradicular teeth contaminated with dual species bacteria of E.faecalis and fusobacterium. 300 samples were pretreated with 100 mM of coaggregation inhibitors (lactose, N-Acetyl-D-galactosamine, L-Arginine, L-Lysine) and disinfection was done with 3% sodium hypochlorite (NaOCl) and 2% chlorhexidine(chx). 15 samples were used as positive control with dual species biofilm. The remaining biovolume and bacterial quantification was analyzed using confocal microscopy and molecular probe assay – rt-PCR(Taqman probe). Results: A significant decrease in the percentage of biovolume and bacterial number after pretreatment with 100 mMol of L-Arginine followed by N-Acetyl galactosamine before 3% NaOCl disinfection compared to 3% NaOCl without pretreatment and positive control (no treatment). (p≤0.05) Conclusion: Coaggregation inhibitors enhanced the antimicrobial activity of 3% sodium hypochlorite and 2% Chlorhexidine. L-Arginine pretreatment before disinfection with 3% NaOCl showed the least percentage of bacterial volume and number of dual species of E.faecalis and F.nucleatum.

Keywords: Sodium hypochlorite, coaggregation inhibitors, E.faecalis, F.nucleatum, Rt-PCR.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )