THE ROLE OF COAGGREGATION INHIBITORS IN ENHANCING THE ANTIBACTERIAL EFFECT OF IRRIGANTS INSIDE THE ROOT CANAL- AN IN VITRO STUDY USING CONFOCAL AND MOLECULAR PROBE ASSAY
*Dr. Hannah Rosaline, MDS, Dr. Deivanayagam Kandswamy, BDS, MDS, FDS, Dr. J. Kalyani, MBBS, DGO, MD, Dr. Velayutham Gopi Krishna, BDS, MDS, PhD and Dr. Chinambedu D. Priya, M.Sc, PhD
Introduction: Root canal infections are polymicrobial. Coaggregation between bacteria in the root canal play a potential role in endodontic infection. The aim of this study was to investigate the antibacterial efficacy of 3% sodium hypochlorite and 2% chlorhexidine pretreated with coaggregation inhibitors against dual species biofilm of E.faecalis and F.nucleatum. Methods: Dentine samples from human uniradicular teeth contaminated with dual species bacteria of E.faecalis and fusobacterium. 300 samples were pretreated with 100 mM of coaggregation inhibitors (lactose, N-Acetyl-D-galactosamine, L-Arginine, L-Lysine) and disinfection was done with 3% sodium hypochlorite (NaOCl) and 2% chlorhexidine(chx). 15 samples were used as positive control with dual species biofilm. The remaining biovolume and bacterial quantification was analyzed using confocal microscopy and molecular probe assay – rt-PCR(Taqman probe). Results: A significant decrease in the percentage of biovolume and bacterial number after pretreatment with 100 mMol of L-Arginine followed by N-Acetyl galactosamine before 3% NaOCl disinfection compared to 3% NaOCl without pretreatment and positive control (no treatment). (p≤0.05) Conclusion: Coaggregation inhibitors enhanced the antimicrobial activity of 3% sodium hypochlorite and 2% Chlorhexidine. L-Arginine pretreatment before disinfection with 3% NaOCl showed the least percentage of bacterial volume and number of dual species of E.faecalis and F.nucleatum.
Keywords: Sodium hypochlorite, coaggregation inhibitors, E.faecalis, F.nucleatum, Rt-PCR.
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