ANALYSIS OF PHENYL PROPANOIDS PROFILE IN THREE POLYGONUM SPECIES BY HPTLC.

B. Ezhilan and R. Neelamegam*

ABSTRACT

HPTLC analysis was carried out on phenyl propanoid compounds profile in the whole-plant samples of selected Polygonum species (P. chinense, P. glabrum and P. barbatum). The methanol extract of whole-plant samples obtained from Polygonum species (P. chinense, P. glabrum and P. barbatum) showed 9, 11 and 7 compounds, respectively, and were compared with eugenol standard. Among the compounds, 3 compounds in each sample were identified as phenyl propanoids while the others were unknown. One unknown and one phenyl propanoid compounds each from P. chinense and P. glabrum showing same peak Rf values (0.01/0.16, respectively). Similarly, another one unknown compound of P. chinense and P. barbatum also showed same peak Rf values (0.97, respectively), while all other detected compounds of Polygonum species showed no similarities in their peak Rf values.

Keywords: HPTLC analysis, Phenyl propanoids, whole-plant material, Methanol extracts, Polygonum species.