EFFECT OF SALINE SOLUTION AND OSMOTIC PRESSURE ON INVERTASE EXTRACTION FROM CELL WALL OF BAKER’S YEAST
*Prof. Michele Vitolo
This work dealt with the autolysis of yeast cell wall carried out with different kinds of salts (sodium bicarbonate, sodium chloride and potassium chloride) and at osmotic pressured varying between 2.0atm and 12.0atm. Through an analysis of variance (test “F”) it was verified that the type of salt had a significant effect on invertase extraction, meanwhile the osmotic pressure, at least at the interval studied, had no significant effect. The highest specific invertase activity (0.660mgRS/mg prot.min) and total invertase activity (60.2mgRS/min) occurred in presence of 0.166M NaHCO3 and 0.042M KCl, respectively. The choice of the salt will depend on the destination to be given to the autolyzate, i.e., sodium bicarbonate if the invertase purification is envisaged or potassium chloride if the autolyzate will be used without any further treatment. It was also evidenced the possible use of conductimetry for controlling the invertase release during autolysis.
Keywords: Osmotic pressure, invertase, baker’s yeast, cell wall.
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