EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

EFFECT OF SALINE SOLUTION AND OSMOTIC PRESSURE ON INVERTASE EXTRACTION FROM CELL WALL OF BAKER’S YEAST

*Prof. Michele Vitolo

ABSTRACT

This work dealt with the autolysis of yeast cell wall carried out with different kinds of salts (sodium bicarbonate, sodium chloride and potassium chloride) and at osmotic pressured varying between 2.0atm and 12.0atm. Through an analysis of variance (test “F”) it was verified that the type of salt had a significant effect on invertase extraction, meanwhile the osmotic pressure, at least at the interval studied, had no significant effect. The highest specific invertase activity (0.660mgRS/mg prot.min) and total invertase activity (60.2mgRS/min) occurred in presence of 0.166M NaHCO3 and 0.042M KCl, respectively. The choice of the salt will depend on the destination to be given to the autolyzate, i.e., sodium bicarbonate if the invertase purification is envisaged or potassium chloride if the autolyzate will be used without any further treatment. It was also evidenced the possible use of conductimetry for controlling the invertase release during autolysis.

Keywords: Osmotic pressure, invertase, baker’s yeast, cell wall.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )