EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

PLASMID PROFILE ANALYSIS AND CURING OF MULTIDRUG RESISTANT BACTERIA ISOLATED FROM HOSPITALS WASTE DUMP SITE IN CALABAR METROPOLIS, NIGERIA

*I. E. Andy and E. A. Okpo

ABSTRACT

This study investigated the plasmid profile analysis and curing of multidrug resistant bacteria isolated from hospitals waste dump site in Calabar metropolis. Samples collection, identification of bacterial isolates, antibiotic susceptibility of the isolates and multiple antibiotic resistance (MAR) index were done using standard techniques. Plasmid DNA extraction were done using zyppy plasmid miniprep kits. Curing of multidrug resistant bacteria isolates was done using acridine orange and cured isolates were re-subjected to antibiotic susceptibility testing. Replicate data were analysed using analysis of variance and student t-test. The bacteria isolated from samples were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus spp, Salmonella spp, Klebsiella pneumoniae, Providencia spp, Enterobacter aerogenes, Proteus spp, Chryseobacterium spp, Bacillus cereus and Serratia marcescens. There was no significant variation in the number of bacterial isolates from the difference sampling points (p>0.05). Bacteria isolates that showed resistance to at least one member from the three classes of antibiotics used were selected as the multidrug resistance isolates and they were Pseudomonas aeruginosa, Providencia sp, Chryseobacterium sp, Providencia rettgeri, Escherichia coli and Staphylococcus aureus. Their MAR index ranged from 0.8-1.0. The selected MDR isolates were all positive for plasmid DNA with sizes greater than 15kbp. It was observed that after curing, the MDR isolates were sensitive to all the antibiotics they were resistance to, which indicates that their resistance were borne on plasmid. The high prevalence of multidrug resistant bacteria and their various resistance pattern observed in the study represents a potential health risk.

Keywords: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa.


[Full Text Article]

INDEXING

NEWS & UPDATION

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 December 2021 Issue has been Published, Kindly check it on http://www.ejpmr.com/home/current_issues

  • EJPMR: DECEMBER ISSUE PUBLISHED

    DECEMBER Issue has been successfully launched on 1 December 2021.

  • EJPMR New Impact Factor

    Its our Pleasure to Inform you that EJPMR Impact Factor has been increased from  5.804 to 6.222, due to high quality Publication at International Level

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 79.57, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )